![]() Advantages with this method include the ability to image large volumes of tissue with EM resolution. Once in the SEM, software is used to find and image the same area of interest on all of the sections (Fig. The sections are collected manually onto a substrate (e.g., a slide or coverslip) or onto a tape using an automated system, such as the ATUMtome. A diamond knife is used in an ultramicrotome to produce thin sections of a resin embedded sample. Serial section array tomography ( Micheva & Smith, 2007) is based on similar principals to serial section TEM but has the advantage that many thousands of sequential sections can be imaged. The best data can be obtained from a stage that has a full 90° tilt range. At least 3 image sets should be captured at different tilt angles. The sample is then tilted and the process repeated. The sample is rotated and an image captured every few degrees until the full 360° is covered. Stereoscopy can be used on any SEM with a tilting stage.įigure 3 shows a diagram demonstrating how an image series can be captured for photogrammetry. Vertical or overlapping details are often lost or distorted using this technique and data is restricted to the surface of the specimen, but this is a good method for obtaining topographical information. A digital model of the surface of a specimen can be created from stereoscopic images using specialist software, which allows z axis measurements to be made ( Hughes et al., 2005). The images are then tinted (red-green or cyan-maroon) and aligned. Stereograms are created by taking two images of the same area, tilting the specimen 5-10° between each image. 2) that can be viewed with anaglyph glasses. The images can by coloured and combined to create a 3D anaglyph stereogram (Fig. ![]() Stereoscopy is the process by which two slightly different images are presented to each eye, creating an illusion of depth in the image. ![]() This article guides you through the available techniques. Some of which can be done on any microscope and some that require specialist instrumentation, software, or microscopes in order to achieve that result. However, there are several SEM techniques that can obtain 3D information about a sample. While the resultant images appear to be three dimensional (3D), they actually contain no depth information. 1 shows a backscattered electron micrograph of daisy pollen grains. ![]()
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